학술논문
Detection of mouse hepatitis virus in mouse colonies using the nested polymerase chain reaction
Document Type
article
Source
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. August 2000 52(4)
Subject
Language
English
ISSN
0102-0935
Abstract
A reverse transcriptase polymerase chain reaction (RT-PCR) to detect mouse hepatitis virus (MHV) in hepatic tissue was developed. To circumvent possible failures in RT-PCR amplifications, a second round of PCR with internal primers was used to confirm the specificity and increase the sensitivity of the test. Using this method specific amplification of MHV sequences was observed in 18 out of 20 mouse colonies examined.