학술논문
Antiretroviral-naive and -treated HIV-1 patients can harbour more resistant viruses in CSF than in plasma.
Document Type
article
Author
Soulie, Cathia; Descamps, Diane; Grudé, Maxime; Schneider, Véronique; Trabaud, Mary-Anne; Morand-Joubert, Laurence; Delaugerre, Constance; Montes, Brigitte; Barin, Francis; Ferre, Virginie; Raymond, Stéphanie; Jeulin, Hélène; Alloui, Chakib; Yerly, Sabine; Pallier, Coralie; Reigadas, Sandrine; Signori-Schmuck, Anne; Guigon, Aurélie; Fafi-Kremer, Samira; Haïm-Boukobza, Stéphanie; Mirand, Audrey; Maillard, Anne; Vallet, Sophie; Roussel, Catherine; Assoumou, Lambert; Calvez, Vincent; Flandre, Philippe; Marcelin, Anne-Geneviève; ANRS Resistance AC11 Group
Source
The Journal of antimicrobial chemotherapy. 70(2)
Subject
Language
Abstract
OBJECTIVES: The neurological disorders in HIV-1-infected patients remain prevalent. The HIV-1 resistance in plasma and CSF was compared in patients with neurological disorders in a multicentre study. METHODS: Blood and CSF samples were collected at time of neurological disorders for 244 patients. The viral loads were >50 copies/mL in both compartments and bulk genotypic tests were realized. RESULTS: On 244 patients, 89 and 155 were antiretroviral (ARV) naive and ARV treated, respectively. In ARV-naive patients, detection of mutations in CSF and not in plasma were reported for the reverse transcriptase (RT) gene in 2/89 patients (2.2%) and for the protease gene in 1/89 patients (1.1%). In ARV-treated patients, 19/152 (12.5%) patients had HIV-1 mutations only in the CSF for the RT gene and 30/151 (19.8%) for the protease gene. Two mutations appeared statistically more prevalent in the CSF than in plasma: M41L (P=0.0455) and T215Y (P=0.0455). CONCLUSIONS: In most cases, resistance mutations were present and similar in both studied compartments. However, in 3.4% of ARV-naive and 8.8% of ARV-treated patients, the virus was more resistant in CSF than in plasma. These results support the need for genotypic resistance testing when lumbar puncture is performed.