학술논문
Iron restriction inside macrophages regulates pulmonary host defense against Rhizopus species.
Document Type
article
Author
Andrianaki, Angeliki M; Kyrmizi, Irene; Thanopoulou, Kalliopi; Baldin, Clara; Drakos, Elias; Soliman, Sameh SM; Shetty, Amol C; McCracken, Carrie; Akoumianaki, Tonia; Stylianou, Kostas; Ioannou, Petros; Pontikoglou, Charalampos; Papadaki, Helen A; Tzardi, Maria; Belle, Valerie; Etienne, Emilien; Beauvais, Anne; Samonis, George; Kontoyiannis, Dimitrios P; Andreakos, Evangelos; Bruno, Vincent M; Ibrahim, Ashraf S; Chamilos, Georgios
Source
Nature communications. 9(1)
Subject
Language
Abstract
Mucormycosis is a life-threatening respiratory fungal infection predominantly caused by Rhizopus species. Mucormycosis has incompletely understood pathogenesis, particularly how abnormalities in iron metabolism compromise immune responses. Here we show how, as opposed to other filamentous fungi, Rhizopus spp. establish intracellular persistence inside alveolar macrophages (AMs). Mechanistically, lack of intracellular swelling of Rhizopus conidia results in surface retention of melanin, which induces phagosome maturation arrest through inhibition of LC3-associated phagocytosis. Intracellular inhibition of Rhizopus is an important effector mechanism, as infection of immunocompetent mice with swollen conidia, which evade phagocytosis, results in acute lethality. Concordantly, AM depletion markedly increases susceptibility to mucormycosis. Host and pathogen transcriptomics, iron supplementation studies, and genetic manipulation of iron assimilation of fungal pathways demonstrate that iron restriction inside macrophages regulates immunity against Rhizopus. Our findings shed light on the pathogenetic mechanisms of mucormycosis and reveal the role of macrophage-mediated nutritional immunity against filamentous fungi.