학술논문
Differences in neutralizing antibody sensitivities and envelope characteristics indicate distinct antigenic properties of Nigerian HIV-1 subtype G and CRF02_AG
Document Type
Report
Author
Wieczorek, Lindsay; Chang, David; Sanders-Buell, Eric; Zemil, Michelle; Martinez, Elizabeth; Schoen, Jesse; Chenine, Agnes-Laurence; Molnar, Sebastian; Barrows, Brittani; Poltavee, Kultida; Charurat, Man E.; Abimiku, Alash'le; Blattner, William; Iroezindu, Michael; Kokogho, Afoke; Michael, Nelson L.; Crowell, Trevor A.; Ake, Julie A.; Tovanabutra, Sodsai; Polonis, Victoria R.; Bartolanzo, Danielle; Reynolds, Alexus; Song, Katherine; Milazzo, Mark; Francisco, Leilani; Mankiewicz, Shauna; Schech, Steven; Golway, Alexandra; Omar, Badryah; Mebrahtu, Tsedal; Lee, Elizabeth; Bohince, Kimberly; Parikh, Ajay; Hern, Jaclyn; Duff, Emma; Lombardi, Kara; Imbach, Michelle; Eller, Leigh Anne; Kibuuka, Hannah; Semwogerere, Michael; Naluyima, Prossy; Zziwa, Godfrey; Tindikahwa, Allan; Mutebe, Hilda; Kafeero, Cate; Baghendaghe, Enos; Lwebuge, William; Ssentogo, Freddie; Birungi, Hellen; Tegamanyi, Josephine; Wangiri, Paul; Nabanoba, Christine; Namulondo, Phiona; Tumusiime, Richard; Musingye, Ezra; Nanteza, Christina; Wandege, Joseph; Waiswa, Michael; Najjuma, Evelyn; Maggaga, Olive; Kenoly, Isaac Kato; Mukanza, Barbara; Maswai, Jonah; Langat, Rither; Ngeno, Aaron; Korir, Lucy; Langat, Raphael; Opiyo, Francis; Kasembeli, Alex; Ochieng, Christopher; Towett, Japhet; Kimetto, Jane; Omondi, Brighton; Leelgo, Mary; Obonyo, Michael; Rotich, Linner; Tonui, Enock; Chelangat, Ella; Kapkiai, Joan; Wangare, Salome; Kesi, Zeddy Bett; Ngeno, Janet; Langat, Edwin; Labosso, Kennedy; Rotich, Joshua; Cheruiyot, Leonard; Changwony, Enock; Bii, Mike; Chumba, Ezekiel; Ontango, Susan; Gitonga, Danson; Kiprotich, Samuel; Ngtech, Bornes; Engoke, Grace; Metet, Irene; Airo, Alice; Kiptoo, Ignatius; Owuoth, John; Sing'oei, Valentine; Rehema, Winne; Otieno, Solomon; Ogari, Celine; Modi, Elkanah; Adimo, Oscar; Okwaro, Charles; Lando, Christine; Onyango, Margaret; Aoko, Iddah; Obambo, Kennedy; Meyo, Joseph; Suja, George; Adamu, Yakubu; Azuakola, Nnamdi; Asuquo, Mfreke; Tiamiyu, Abdulwasiu Bolaji; Mohammed, Samirah Sani; Okoye, Ifeanyi; Odeyemi, Sunday; Suleiman, Aminu; Umejo, Lawrence; Enas, Onome; Mbachu, Miriam; Chigbu-Ukaegbu, Ijeoma; Adai, Wilson; Odo, Felicia Anayochukwu; Abdu, Rabi; Akiga, Rosemary; Nwandu, Helen; Okolo, CHisara; Okeke, Ndubuisis; Parker, Zahra; Linus, Asogwa Ugochukwu; Agbaim, Concilia Amaka; Adegbite, Tunde; Harrison, Nkenchiere; Adelakun, Adewale; Chioma, Ekeocha; Idi, Victoria; Eluwa, Rachel; Nwalozie, Jumoke; Faith, Igiri; Okanigbuan, Blessing; Emmanuel, Achugwo; Nnadi, Nkiru; Rosemary, Ndubuisi; Natalie, Uzoegwu Amaka; Owanza, Obende Theresa; Francis, Falaju Idowu; Elemere, Jacintal; Lauretta, Obilor Ifeoma; Akinwale, Edward; Ochai, Inalegwu; Maganga, Lucas; Bahemana, Emmanuel; Khamadi, Samoel; Njegite, John; Lueer, Connie; Kisinda, Abisai; Mwamwaja, Jaquiline; Mbwayu, Faraja; David, Gloria; Mwaipopo, Mtasi; Gervas, Reginald; Mkondoo, Doroth; Somi, Nancy; Kiliba, Paschal; Mwaisanga, Gwamaka; Msigwa, Johnisius; Mfumbulwa, Hawa; Edwin, Peter; Olomi, Willyhelmina; Charurat, Manhattan; Abayomi, Aka; Adebajo, Sylvia; Baral, Stefan; Gaydos, Charlotte; Hu, Fengming; Malia, Jennifer; Nowak, Rebecca; Ononaku, Uchenna; Peel, Sheila; Ramadhani, Habib; Robb, Merlin; Rodriguez-Hart, Cristina; Shoyemi, Elizabeth; Tiamiyu, Abdulwasiu; Vasan, Sandhya
Source
Virology Journal. June 29, 2024, Vol. 21 Issue 1
Subject
Language
English
ISSN
1743-422X
Abstract
The magnitude of the HIV-1 epidemic in Nigeria is second only to the subtype C epidemic in South Africa, yet the subtypes prevalent in Nigeria require further characterization. A panel of 50 subtype G and 18 CRF02_AG Nigerian HIV-1 pseudoviruses (PSV) was developed and envelope coreceptor usage, neutralization sensitivity and cross-clade reactivity were characterized. These PSV were neutralized by some antibodies targeting major neutralizing determinants, but potentially important differences were observed in specific sensitivities (eg. to sCD4, MPER and V2/V3 monoclonal antibodies), as well as in properties such as variable loop lengths, number of potential N-linked glycans and charge, demonstrating distinct antigenic characteristics of CRF02_AG and subtype G. There was preferential neutralization of the matched CRF/subtype when PSV from subtype G or CRF02_AG were tested using pooled plasma. These novel Nigerian PSV will be useful to study HIV-1 CRF- or subtype-specific humoral immune responses for subtype G and CRF02_AG. Keywords: HIV, Envelope (Env), Antibody, Nigeria, West Africa, Subtype G, CRF02_AG, Neutralization
Author(s): Lindsay Wieczorek[sup.1,2] , David Chang[sup.1,2,7] , Eric Sanders-Buell[sup.1,2] , Michelle Zemil[sup.1,2] , Elizabeth Martinez[sup.1,2] , Jesse Schoen[sup.1,2] , Agnes-Laurence Chenine[sup.1,2,8] , Sebastian Molnar[sup.1,2] , Brittani Barrows[sup.1,2,9] , Kultida Poltavee[sup.3] [...]
Author(s): Lindsay Wieczorek[sup.1,2] , David Chang[sup.1,2,7] , Eric Sanders-Buell[sup.1,2] , Michelle Zemil[sup.1,2] , Elizabeth Martinez[sup.1,2] , Jesse Schoen[sup.1,2] , Agnes-Laurence Chenine[sup.1,2,8] , Sebastian Molnar[sup.1,2] , Brittani Barrows[sup.1,2,9] , Kultida Poltavee[sup.3] [...]