부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.peptides.2007.11.019 Byline: Hayet Soualmia (a), Fekri Abroug (b), Yasmina Djeridane (c) Keywords: Atrial natriuretic peptide; Scorpion toxin; 6-Hydroxydopamine; Rat; Heart Abstract: Scorpion envenomation is considered public health problem in Northern African countries. The mechanisms of cardiac dysfunction following scorpion envenomation are not fully understood. This study examined the effect and mechanisms underlying scorpion toxin action from Androctonus australis garzonii on atrial natriuretic peptide (ANP) release from rat atrium using in vitro organ perifusion. Male Sprague Dawley rats were used in this study. Three experiments were conducted. In experiment 1, atrial tissues were exposed either to Krebs-bicarbonate buffer medium (control) or to scorpion toxin (10.sup.-8 M to 10.sup.-6 M). In experiment 2, animals were chemically sympathectomized with a single intraperitoneal injection of 6-hydroxydopamine (6-OHDOPA) at a dose of 40[mu]g/g 24h before sacrifice. Vehicle-treated rats served as control. Atrial tissues were collected and perifused in the presence of 10.sup.-6 M scorpion toxin. In experiment 3, atrial tissues were exposed to 10.sup.-6 M scorpion toxin either in the absence or presence of 10.sup.-6 M propranolol (a [beta]-adrenoceptor blocker), or 10.sup.-6 M tetrodotoxin (a sodium channel blocker). ANP levels released in the perifusion medium were determined by radioimmunoassay. The scorpion toxin at 10.sup.-6 M induced a significant (p Author Affiliation: (a) Institut Superieur des Technologies Medicales, Tunis, Tunisia (b) CHU Monastir, Service de Reanimation Polyvalente et Laboratoire d'Experimentation Animale, Monastir, Tunisia (c) Mecanismes et Physiopathologie des Rythmes Circadiens et INSERM U713, Faculte de Medecine Pierre et Marie Curie, 75013 Paris, France Article History: Received 26 September 2007; Revised 27 November 2007; Accepted 29 November 2007