학술논문
Detection of clonal plasma cells in POEMS syndrome using multiparameter flow cytometry
Document Type
article
Author
Arata Ishii; Shokichi Tsukamoto; Naoya Mimura; Yurie Miyamoto-Nagai; Yusuke Isshiki; Shinichiro Matsui; Sanshiro Nakao; Asuka Shibamiya; Yutaro Hino; Kensuke Kayamori; Nagisa Oshima-Hasegawa; Tomoya Muto; Yusuke Takeda; Tomoki Suichi; Sonoko Misawa; Chikako Ohwada; Koutaro Yokote; Satoshi Kuwabara; Chiaki Nakaseko; Hiroyuki Takamatsu; Emiko Sakaida
Source
Scientific Reports, Vol 14, Iss 1, Pp 1-9 (2024)
Subject
Language
English
ISSN
2045-2322
Abstract
Abstract POEMS syndrome (polyneuropathy, organomegaly, endocrinopathy, monoclonal protein [M-protein], and skin changes) is a rare systemic disorder characterized by various symptoms caused by underlying plasma cell (PC) dyscrasia. Detection of monoclonal PCs is mandatory for the diagnosis of POEMS syndrome; however, the usefulness of EuroFlow-based next-generation flow cytometry (EuroFlow-NGF) in POEMS syndrome for detecting monoclonal PCs in bone marrow (BM) and the gating strategy suitable for flow cytometry study of POEMS syndrome remain unknown. We employed EuroFlow-NGF-based single-tube eight-color multiparameter flow cytometry (MM-flow) and established a new gating strategy (POEMS-flow) to detect the monoclonal PCs in POEMS syndrome, gating CD38 broadly from dim to bright and CD45 narrowly from negative to dim compared to MM-flow. MM-flow detected monoclonal PCs in 9/25 (36.0%) cases, including 2/2 immunofixation electrophoresis (IFE)-negative cases (100%). However, POEMS-flow detected monoclonal PCs in 18/25 cases (72.0%), including 2/2 IFE-negative cases (100%). POEMS-flow detected monoclonal PCs with immunophenotypes of CD19− in 17/18 (94.4%). In six cases where post-treatment samples were available, the size of the clones was significantly reduced after the treatment (P = 0.031). POEMS-flow can enhance the identification rate of monoclonal PCs in POEMS syndrome and become a valuable tool for the diagnosis of POEMS syndrome.