학술논문
Establishment of two-dimensional gel electrophoresis for soybean protein isolate and its application
Document Type
article
Author
Source
Grain & Oil Science and Technology, Vol 3, Iss 3, Pp 100-109 (2020)
Subject
Language
English
ISSN
2590-2598
Abstract
To optimize the conditions for the establishment of two-dimensional gel electrophoresis (2-DE) of soybean protein isolate (SPI), we investigated Ampholine mixture, anodic and cathodic electrolytes, loading amount of sample, acrylamide concentration, pH gradient and gel staining method in two-dimensional gel electrophoresis to optimize the protein imaging conditions in two-dimensional gel. The results of mixed-level design experiments showed that Ampholine, loading amount and gel staining method had significant effect (P