학술논문

Cell wall polysaccharides hydrolysis of malting barley (Hordeum vulgare L.): a review
Document Type
article
Source
Biotechnologie, Agronomie, Société et Environnement, Vol 15, Iss 2, Pp 301-313 (2011)
Subject
Malting barley
cell walls
polysaccharides
Hordeum vulgare
β-glucan
arabinoxylan
Biotechnology
TP248.13-248.65
Environmental sciences
GE1-350
Language
English
French
ISSN
1370-6233
1780-4507
Abstract
Malting quality results from the different steps of the malting process. Malting uses internal changes of the seed occurring during germination, such as enzymes synthesis, to obtain a good hydrolysis process and the components required. Among the three main hydrolytic events observed, that are namely starch degradation, cell wall breakdown and protein hydrolysis, an efficient cell wall polysaccharides hydrolysis is an essential condition for a final product of quality. Indeed, because of the physical barrier of the cell wall, cell wall polysaccharides hydrolysis is one of the first steps expected from the process to gain access to the cell components. Moreover, viscosity problem and haze formation in malting industry are related to their presence during the process when inefficient degradation occurs, leading to increased production time and cost. Understanding the key elements in cell wall degradation is important for a better control. (1-3,1-4)-β-glucans and arabinoxylans are the main constituents of cell wall. (1-3,1-4)-β-glucans are unbranched chains of β-D-glucopyranose residues with β-(1,3) linkages and β-(1,4) linkages. Arabinoxylan consists in a backbone of D-xylanopyranosyl units linked by β-(1-4) bonds connected to single L-arabinofuranose by α-(1→2) or α-(1→3)-linkages. Degradation of (1-3,1-4)-β-glucans is processed by the (1-3,1-4)-β-glucanases, the β-glucosidases and the β-glucane exohydrolases. It seems that the (1-3)-β-glucanases are also involved. Arabinoxylans are mainly decomposed by (1-4)-β-xylan endohydrolase, arabinofuranosidase and β-xylosidase.