학술논문
不同叶果比对设施'红美人'杂柑秋梢叶片生理生化特征的影响 / Effects of leaf/fruit ratios on physiological and biochemical characteristics of autumn shoot leaves of'Hongmeiren'citrus hybrid under protected cultivation
Document Type
Academic Journal
Author
王鹏; 金龙飞; 黄贝; 刘烽; 温明霞; 吴韶辉; 徐建国; WANG Peng; JIN Longfei; HUANG Bei; LIU Feng; WEN Mingxia; WU Shaohui; XU Jianguo
Source
华中农业大学学报 / Journal of Huazhong Agricultural University. 43(1):133-140
Subject
Language
Chinese
ISSN
1000-2421
Abstract
为探究不同叶果比(leaf fruit ratio,LFR)对设施柑橘秋梢叶片生理生化特征的影响,以设施栽培'红美人'杂柑为试材,设置高(LFR=100)、中(LFR=80)、低(LFR=60)3个叶果比,测定秋梢叶片中矿质元素、碳氮代谢物、碳氮代谢的关键酶、抗氧化酶活性等生理指标的变化.结果显示,LFR80和LFR100秋梢叶片的磷、钾、镁、淀粉含量较高;丙二醛、脯氨酸、抗坏血酸含量较低;β-淀粉酶、硝酸还原酶、谷氨酰胺合成酶和抗坏血酸过氧化物酶的活性较高;LFR80和LFR100秋梢叶片整体养分含量充足,受胁迫程度较低.结果表明,设施栽培'红美人'杂柑的叶果比控制在80～100有利用于平衡营养生长和生殖生长,延缓叶片衰老,维持树势强健.
6-year-old'Hongmeiren'citrus hybrid trees grafted on trifoliate orange(Poncirus trifolia-ta)rootstock were used to study the effects of different leaf/fruit ratios on the physiological and biochemical characteristics of autumn shoot leaves of citrus under protected cultivation.Three leaf/fruit ratios including low(LFR60)medium(LFR80)and high(LFR100)were set up.The physiological and biochemical in-dexes including the contents of mineral elements,metabolites of carbon and nitrogen,activities of key en-zymes and antioxidant enzymes in the metabolism of carbon and nitrogen in autumn shoot leaves were deter-mined.The results showed that the content of phosphorus,potassium,magnesium,starch in autumn shoot leaves of LFR80 and LFR100 were significantly higher than that of LFR60.The content of malondialde-hyde,proline,ascorbic acid in autumn shoot leaves of LFR80 and LFR100 were significantly lower than that of LFR60.The activity of β-amylase,nitrate reductase,glutamine synthetase,ascorbate peroxidase in autumn shoot leaves of LFR80 and LFR100 were significantly higher than that of LFR60.The content of nutrients in autumn shoot leaves of LFR80 and LRF100 were more sufficient and the degree of stress was lower.It is indicated that controlling the leaf/fruit ratio of'Hongmeiren'hybrid citrus under protected culti-vation between 80 and 100 is beneficial for balancing vegetative and reproductive growth,delaying leaf se-nescence,and maintaining robust tree.
6-year-old'Hongmeiren'citrus hybrid trees grafted on trifoliate orange(Poncirus trifolia-ta)rootstock were used to study the effects of different leaf/fruit ratios on the physiological and biochemical characteristics of autumn shoot leaves of citrus under protected cultivation.Three leaf/fruit ratios including low(LFR60)medium(LFR80)and high(LFR100)were set up.The physiological and biochemical in-dexes including the contents of mineral elements,metabolites of carbon and nitrogen,activities of key en-zymes and antioxidant enzymes in the metabolism of carbon and nitrogen in autumn shoot leaves were deter-mined.The results showed that the content of phosphorus,potassium,magnesium,starch in autumn shoot leaves of LFR80 and LFR100 were significantly higher than that of LFR60.The content of malondialde-hyde,proline,ascorbic acid in autumn shoot leaves of LFR80 and LFR100 were significantly lower than that of LFR60.The activity of β-amylase,nitrate reductase,glutamine synthetase,ascorbate peroxidase in autumn shoot leaves of LFR80 and LFR100 were significantly higher than that of LFR60.The content of nutrients in autumn shoot leaves of LFR80 and LRF100 were more sufficient and the degree of stress was lower.It is indicated that controlling the leaf/fruit ratio of'Hongmeiren'hybrid citrus under protected culti-vation between 80 and 100 is beneficial for balancing vegetative and reproductive growth,delaying leaf se-nescence,and maintaining robust tree.