학술논문
Deep Characterization of the Human Antibody Response to Natural Infection Using Longitudinal Immune Repertoire Sequencing*
Document Type
Article
Author
Source
Molecular and Cellular Proteomics (MCP Online); February 2020, Vol. 19 Issue: 2 p278-293, 16p
Subject
Language
ISSN
15359476; 15359484
Abstract
We conducted targeted immune repertoire RNA sequencing on flow cytometry-sorted human B cell subsets to longitudinally profile how the healthy human antibody response changes through time and natural infections. Our results indicate that antibodies have stable variable (V) gene usage profiles based on antigen experience. We additionally observed that one VJ gene combination can give rise to more than one complementarity-determining region 3 (CDR3) sequence. Unexpectedly, we also found that isotype switching may occur earlier than previously thought.