학술논문
Cloning and characterisation of the human 5-HT 5Aserotonin receptor
Document Type
Article
Author
Source
FEBS Letters; January 1994, Vol. 355 Issue: 3 p242-246, 5p
Subject
Language
ISSN
00145793
Abstract
The human 5-HT 5Aserotonin receptor has been cloned. As with the mouse and rat 5-HT 5Areceptors, the gene consists of two coding exons separated by a large intron. The deduced amino acid sequence of the gene reveals a protein of 357 residues which shares 93% (nucleotide) and 84% (amino acid) identity to the cloned mouse 5-HT 5Areceptor. We have determined the tissue distribution of the receptor by reverse transcriptase-PCR and found expression in all regions of the brain examined with little or no expression in peripheral tissues. The receptor has been transiently expressed in Cos M6 cells and exhibits a pharmacological profile closely resembling the mouse and rat 5-HT 5Areceptors with high, specific binding for ergotamine and methiothepin.