부산대학교 도서관

Malate stimulated NH3 assimilation, as determined by a (2-oxoglutarate, NH3)-dependent O2 evolution system, by up to 3-fold in chloroplasts isolated from leaves of dicot but not monocot species. This difference was apparently correlated with the endogenous metabolite pools present in these chloroplast preparations. During NH3 assimilation the glutamate and glutamine pools were large in spinach (dicot) but small in oat chloroplasts. The reverse was the case for the 2-oxoglutarate (2-OG) pool. The addition of malate substantially increased the glutamate, glutamine and 2-OG pools in spinach chloroplasts but had little effect in oat chloroplasts. This suggests that the supply of 2-OG was apparently limiting NH3 assimilation in spinach chloroplasts. Malate increased this supply and, consequently, stimulated NH3 assimilation. On the other hand, NH3 assimilation in oat chloroplasts seemed to be limited by the supply of glutamate and glutamine which could not be overcome by the addition of malate. Chloroplasts were also isolated from oat seedlings watered with high nutrient solution. The rates of NH3 assimilation in these organelles exceeded those obtained in spinach chloroplasts. But the addition of malate had little effect on (2-OG, NH3)-dependent O2 evolution in these oat chloroplasts. Since malate did not inhibit this activity it is conceivable that it still might play a role, albeit a 'passive' role, in serving as a counter-ion for 2-OG uptake via the 2-OG translocator and glutamate export via the Dct translocator during NH3 assimilation.