학술논문
Cloning, expression, and nucleotide sequence of a mutant glgC gene from Escherichia coli B
Document Type
Article
Author
Source
Journal of Bacteriology; July 1992, Vol. 174 Issue: 13 p4509-4512, 4p
Subject
Language
ISSN
00219193; 10985530
Abstract
A mutant glgC gene contained in a 10.9-kb PstI fragment was cloned from the Escherichia coli B strain SG5 via colony hybridization by using a wild-type glgC probe. The altered allosteric properties of the expressed ADPglucose synthetase were found to result from the conversion of proline to serine at amino acid residue 295.