학술논문
Expression of Arabidopsis thaliana xylose isomerase gene and its effect on ethanol production in Flammulina velutipes.
Document Type
Article
Source
Subject
*FUNGAL gene expression
*ARABIDOPSIS thaliana
*ISOMERASE genetics
*ETHANOL
*FLAMMULINA velutipes
*PENTOSES
*FERMENTATION
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Language
ISSN
1878-6146
Abstract
Abstract: To improve the pentose fermentation rate in Flammulina velutipes, the putative xylose isomerase (XI) gene from Arabidopsis thaliana was cloned and introduced into F. velutipes and the gene expression was evaluated in transformants. mRNA expression of the putative XI gene and XI activity were observed in two transformants, indicating that the putative gene from A. thaliana was successfully expressed in F. velutipes as a xylose isomerase. In addition, ethanol production from xylose was increased in the recombinant strains. This is the first report demonstrating the possibility of using plant genes as candidates for improving the characteristics of F. velutipes. [Copyright &y& Elsevier]