학술논문
Retrospective Genomic Characterization of a 2017 Dengue Virus Outbreak, Burkina Faso.
Document Type
Journal Article
Author
Letizia, Andrew G.; Pratt, Catherine B.; Wiley, Michael R.; Fox, Anne T.; Mosore, Mba; Agbodzi, Bright; Yeboah, Clara; Kumordjie, Selassie; Paola, Nicholas Di; Assana, Kone Cisse; Coulidiaty, David; Ouedraogo, Casimir; Kofi Bonney, Joseph H.; Ampofo, William; Tarnagda, Zékiba; Sangaré, Lassana; Di Paola, Nicholas; Bonney, Joseph H Kofi
Source
Subject
*IN vitro studies
*DENGUE
*BIOLOGICAL evolution
*FLAVIVIRUSES
*RETROSPECTIVE studies
*SEROTYPES
*EPIDEMICS
*GENOMICS
*
*
*
*
*
*
*
Language
ISSN
1080-6040
Abstract
Knowledge of contemporary genetic composition of dengue virus (DENV) in Africa is lacking. By using next-generation sequencing of samples from the 2017 DENV outbreak in Burkina Faso, we isolated 29 DENV genomes (5 serotype 1, 16 serotype 2 [DENV-2], and 8 serotype 3). Phylogenetic analysis demonstrated the endemic nature of DENV-2 in Burkina Faso. We noted discordant diagnostic results, probably related to genetic divergence between these genomes and the Trioplex PCR. Forward and reverse1 primers had a single mismatch when mapped to the DENV-2 genomes, probably explaining the insensitivity of the molecular test. Although we observed considerable homogeneity between the Dengvaxia and TetraVax-DV-TV003 vaccine strains as well as B cell epitopes compared with these genomes, we noted unique divergence. Continual surveillance of dengue virus in Africa is needed to clarify the ongoing novel evolutionary dynamics of circulating virus populations and support the development of effective diagnostic, therapeutic, and preventive countermeasures. [ABSTRACT FROM AUTHOR]