학술논문
Carboxypeptidase A immobilization with zeolitic imidazolate framework for enhancement of ochratoxin A degradation ability.
Document Type
Article
Author
Source
Subject
*CARBOXYPEPTIDASES
*AGRICULTURAL wastes
*MANUFACTURING processes
*COMPLEX matrices
*FOOD production
*MYCOTOXINS
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Language
ISSN
0954-0105
Abstract
Ochratoxin A (OTA) is a highly toxic potential contaminant of most agricultural products, and OTA is difficult to remove in general food production processes. In this study, we immobilized polyvinyl pyrrolidone-coated carboxypeptidase A (CPA) into zeolitic imidazolate framework material to improve the ability for OTA degradation. In this method, carboxypeptidase A was dispersed well in the material and maintained its stability and ability to degrade OTA, under complex matrix conditions with better repeated use performance than free carboxypeptidase A. The substrate conversion rate of CPA increased 3 times after immobilization. After repeated use of 10 cycles, the composite enzyme still retained 71.37% of its initial activity. The degradation rate of OTA was 30.69% higher than that of free CPA. This method may play an important role in the degradation of mycotoxins. [ABSTRACT FROM AUTHOR]