학술논문

Complex Carbohydrate Histochemistry and Ultracytochemistry of the Sheep Lacrimal Gland.
Document Type
Article
Source
Anatomia, Histologia, Embryologia: Journal of Veterinary Medicine Series C. Feb2000, Vol. 29 Issue 1, p19-24. 6p.
Subject
*LACRIMAL apparatus
*CYTOCHEMISTRY
*HISTOCHEMISTRY
SHEEP anatomy
Language
ISSN
0340-2096
Abstract
The chemical content of the secretion of the sheep lacrimal gland was analysed at the light and electron microscope levels by applying histochemical techniques and an ultrastructural histochemical method (periodic acid, thiocarbohydrazide and silver proteinate). Mucosubstance histochemistry demonstrated acidic glycoconjugates, mainly sulphated, in the mucous and seromucous glandular cells and in the apical portion of the cells lining the terminal ducts. Moreover, secretory granules, stained with PA-TCH-SP, showed a different localization of the reaction product. The presence of lysozyme was also found in the glandular serous cells. These histochemical studies demonstrate that the secretion of sheep lacrimal glands is mixed, having serous, mucous and seromucous components, and that an excellent correlation exists between the secretory granule substructure and glycoprotein localization. [ABSTRACT FROM AUTHOR]