학술논문
Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application.
Document Type
Article
Author
Source
Subject
*DRUG therapy for hyperlipidemia
*POLYSACCHARIDES
*TRIGLYCERIDES
*HDL cholesterol
*MEDICINAL plants
*HERBAL medicine
*ANTILIPEMIC agents
*ULTRASONIC imaging
*TEMPERATURE
*ANIMAL experimentation
*LDL cholesterol
*SUPEROXIDE dismutase
*PROTEOLYTIC enzymes
*DYNAMICS
*MALONDIALDEHYDE
*DESCRIPTIVE statistics
*DOSE-effect relationship in pharmacology
*ANALYTICAL chemistry techniques
*CELLULOSE
*CHINESE medicine
*LIPIDS
*MICE
*CHOLESTEROL
*PHARMACODYNAMICS
*DRUG administration
*DRUG dosage
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Language
ISSN
2314-6133
Abstract
This study focused on the optimization of ultrasound-assisted compound enzyme extraction for polysaccharides (RTPs) from Radix trichosanthis by orthogonal experiment and response surface methodology, and then its extraction kinetics model and antihyperlipidemic activities were studied. The optimum extraction process was as follows: cellulase—1.0%, papain—1.0%, pectase—0.5%, pH—5, extraction temperature—50°C, and liquid-to-solid ratio—30 mL/g; prediction value of RTPs was 7.54%; the experimental yield of RTPs was 7.22%, while 50 minutes was optimized in Weibull kinetics model. Then high-dose groups of RTP extract could reduce the TC, TG, and LDL-C levels and increase the level of HDL-C in high-fat mice, with the ability to lower the MDA content and enhance SOD level. [ABSTRACT FROM AUTHOR]