학술논문
Toscana Virus Genome Stability: Data from a Meningoencephalitis Case in Mantua, Italy.
Document Type
Article
Author
Source
Subject
*ARBOVIRUS diseases
*MENINGOENCEPHALITIS
*GENETICS of virus diseases
*SEROLOGY
*WEST Nile fever diagnosis
*NUCLEOTIDE sequence
*DIAGNOSIS
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Language
ISSN
1530-3667
Abstract
In July of 2013, samples from a patient with a neurological syndrome were collected from Mantua hospital and sent to the National Reference Laboratory for Arboviruses (National Institute of Health, Rome). On the basis of the symptoms, serological and molecular assays were performed to diagnose either West Nile virus (WNV) or Toscana virus (TOSV) infection. Molecular and serological tests confirmed TOSV infection. Virus isolation was obtained from cerebrospinal fluid. A full genome sequence was determined from this TOSV strain with next-generation sequencing using Ion Torrent technology. Nucleotide and amino acidic sequences grouped phylogenetically with lineage TOSV A and showed a low genome variability. [ABSTRACT FROM AUTHOR]