학술논문
Simultaneous Measurements of Creatinine and Purine Derivatives in Ruminant's Urine Using Ion-Pair HPLC.
Document Type
Article
Source
Subject
*URINALYSIS
*CHROMATOGRAPHIC analysis
*SHEEP feeding
*MICROBIAL proteins
*RUMINANT feeding & feeds
*CREATININE
*URIC acid
*PURINES
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Language
ISSN
1082-6076
Abstract
A new and sensitive ion-pair RP-HPLC method was developed for concomitant measurement of the creatinine and purine derivatives (PD): allantoin, uric acid, and hypoxanthine using allopurinol as internal standard. Urine was chromatographed on a Symmetry C18 column, using UV detection. Elution with a gradient of methanol in potassium phosphate buffer, pH = 4, containing 3 mM 1-octane-sulfonic acid, sodium salt yielded sharp, well-resolved peaks within 25 min. Application of this method permits the precise determination of these compounds in sheep urine. [ABSTRACT FROM AUTHOR]