학술논문

Novel and toxin-producing epiphytic dinoflagellates isolated from sub-tropical Raoul Island, Kermadec Islands group.
Document Type
Article
Source
New Zealand Journal of Marine & Freshwater Research. Dec2014, Vol. 48 Issue 4, p594-599. 6p.
Subject
*DINOFLAGELLATES
*EPIPHYTIC lichens
*PALYTOXIN
*SPECIES
Language
ISSN
0028-8330
Abstract
Epiphytic dinoflagellates were isolated from samples collected from macroalgae and corals during an expedition to the Kermadec Islands group, led by the Auckland Museum in November 2013. Isolates included species ofGambierdiscusandOstreopsis, genera included in the ciguatera fish poisoning complex. Cells of a compressed (apical to antapical) form ofGambierdiscuswere isolated into growth media, but species identification was not possible as isolates failed to grow in culture. TheOstreopsisisolate was successfully cultured and appears to be a new species (a full description will be undertaken). Palytoxin production was determined by chemical analysis (LC-MS/MS) as 0.013 pg palytoxin equiv. per cell. Prorocentrum isolates were identified morphologically asP. cf.emarginatumandP. lima; both were non-toxic.Amphidiniumcf.massartiiandCoolia malayensiswere identified by DNA sequence analysis. The suite of dinoflagellates isolated was typical of the co-occurring genera found throughout the Pacific region. [ABSTRACT FROM AUTHOR]