부산대학교 도서관

BACKGROUND:: Endogenous induction of cartilage differentiation is to use a certain carrier to integrate the target genes into stem cells to induce self-differentiation by self-induced secretion of cytokines. OBJECTIVE:: To observe the ability of rabbit bone marrow mesenchymal stem cells (BMSCs) transfected with transforming growth factor beta 3 (TGF-β3) to differentiate into cartilage phenotype through adeno-associated virus vector. METHODS:: The third passage BMSCs in logarithmic growth phase was transfected by rAAV2-TGF-β3. Total protein was extracted from cells at 3, 6, 9, 12 days after transfection, and TGF-β3 was detected through ELISA. After 2 weeks of culturing, mRNA expressions of type II collagen were determined by RT-PCR and the collagen II protein was detected by western blot. The expression of proteoglycan was shown by toluidine blue staining. RESULTS AND CONCLUSION:: After transfection, BMSCs can express TGF-β3 stably, and make a better cartilage transformation compared to negative control group. TGF-β3 can be transfected into BMSCs through adeno-associated virus vectors and induce cell differentiation into cartilage phenotype.Sui LJ, Lü CY, Zhang HN, Xu H, Gao ZY, Sui AH, Wang YZ. Differentiation of rabbit mesenchymal stem cells into cartilage phenotype induced by transforming growth factor beta 3.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2011;15(27): 5048-5052. [http://www.crter.cn http://en.zglckf.com]