학술논문
Prediction and Identification of an Acid-inducible Promoter from Lactococcus lactis ssp. cremoris MG1363
Document Type
Article
Author
Source
Food Science and Biotechnology, 24(5), pp.1749-1753 Oct, 2015
Subject
Language
English
ISSN
2092-6456
1226-7708
1226-7708
Abstract
Using a promoter online prediction system, the gene sequence of GroEL protein from Lactococcus lactis ssp. cremoris MG1363 was analysed and a putative promoter, designated as gro, was further studied. Under acidic conditions, gro showed preliminary promoter function by driving the expression of lacZ reporter gene in Escherichia coli DH5α. In addition, gro drove the gene expression of GroEL and green fluorescent protein. In this paper, an acid-inducible promoter was successfully identified. This promoter has a wide prospect in the field of biotechnology.