부산대학교 도서관

Humoral immune responses of mice against sheep red blood cell (SRBC, T cell dependent antigen) and Polyvinylpyrrolidone (PVP, T cell independent antigen) were compared at various ambient temperatures. Mice acclimatized to 25°C (Balb/c mice and C57BL/6 mice) were immunized by intraperitoneal and intravenous injection of SRBC or PVP, and immediately after that, they were transferred to and kept in cold (7°C), moderate (25°C) or hot (37°C) climatic chambers. Five days later, these mice were sacrificed and splenic anti-SRBC or anti-PVP IgM plapue forming cells (IgM PFC) and serum haemaggulutinating antibody (HA) titers were measured. Delayed type hypersensitivity reaction against SRBC was also measured as the parameter of cell-mediated immune response in Balb/c mice. Results were as follows;1) Mice anti-SRBC IgM response at low ambient temperature was significantly enhanced in C57BL/6 mice compared with that at moderate temperature, while no remarkable change was found in Balb/c mice.2) In contrast, the enhancing effect of the low ambient temperature was not exhibited on the IgM PFC response against T cell independent antigen, PVP, in C57BL/6 mice.3) At high ambient temperature, anti-SRBC IgM responses were depressed in both C57BL/6 and Balb/c mice, and depressed anti-PVP IgM response were also observed in C57BL/6 mice.4) Delayed type hypersensitivity reaction against SRBC determined by measurement of hind foot pad swelling was not changed at each temperature in Balb/c mice.These results suggest that enhancing effect of the low ambient temperature on mice IgM immune response may be mediated through regulatory T cells (helper T cells or suppressor T cells), whereas macrophages or B cells may be responsible for suppressing effect of the high ambient temperature.