부산대학교 도서관

As a result of an quality control evaluation of blood culture at our hospital, we found that the contamination rate was high. Thus, to lower the contamination rate and conduct a highly reliable test, we revised the blood culture sampling manual. The investigation period was from April 2016 to September 2017. We compared the contamination rate between the period from April to September 2016 (hereinafter referred to as before improvement) and the period from April to September 2017 (hereinafter referred to as after improvement) excluding the transition period. The disinfectant indicated in our manual before improvement was 10% povidone iodine or alcohol. However, after improvement, we started to disinfect the skin with alcohol and then with 1% chlorhexidine alcohol. Along with this change, we revised the reaction time written in the manual. Also, we revised the appropriate amount of blood from 5 to 10 mL in the manual. The contamination rate before improvement was 17.4%, and after improvement, it decreased to 4.8%. Also, the positivity rate before improvement was 22.4%, and after improvement, it decreased to 15.1%. In addition, the number of strains detected before improvement was 466, and after improvement, it decreased to 266. CNS, which is a representative of bacterial contaminants, decreased markedly in positivity rate from 44.8% to 21.8%. It is considered that altering the skin disinfection method for the blood sampling site and correctly performing the disinfection operations were effective in reducing the contamination at the time of blood collection.