부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.virol.2008.12.035 Byline: Kening Wang (a), Gowtham Mahalingam (a), Yumi Imai (b), Lesley Pesnicak (a), Todd T. Margolis (b), Stephen E. Straus (a), Jeffrey I. Cohen (a) Keywords: Herpes simplex virus 2; Tissue specific gene expression; Latency-associated transcript; Transgenic mouse; In situ hybridization; Neuron Abstract: We performed in situ hybridization to determine the cell type specific accumulation of the intron of the latency-associated transcript (LAT) in tissues in HSV-2 LAT transgenic mice in which LAT expression is driven by its native promoter. We identified LAT in multiple cell types in most tissues analyzed from HSV-2 LAT transgenic mice. While weak to moderate signals were seen in brain and spinal cord neurons, epithelial cells, and muscle cells, the strongest signals were detected in neurons from dorsal root and trigeminal ganglia. About 70-86% of neurons in these ganglia were LAT-positive with varying signal intensities, while cells surrounding the neurons were LAT-negative. The frequency of A5 or KH10-positive neurons was similar in LAT-positive and total neurons. These data indicate that HSV-2 LAT promoter activity is not restricted to neurons and that LAT accumulation in ganglionic neurons is likely regulated by cell-specific factors. Author Affiliation: (a) Medical Virology Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, 10 Center Drive, Room 11N234 Bethesda, MD 20892, USA (b) F.I. Proctor Foundation and Department of Ophthalmology, University of California, San Francisco, California, USA Article History: Received 25 September 2008; Revised 18 October 2008; Accepted 22 December 2008