학술논문
Isolation of a novel plasmid, pNi15, from Enterobacter sp. Ni15 containing a nickel resistance gene
Document Type
Author abstract
Source
FEMS Microbiology Letters. April, 2006, Vol. 257 Issue 2, p177, 5 p.
Subject
Language
English
ISSN
0378-1097
Abstract
To purchase or authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1574-6968.2006.00130.x Byline: Young-Keun Lee, Hwa-Hyoung Chang, Ho-Jin Lee, Heesoon Park, Kyung Hee Lee, Min-Ho Joe Keywords: Enterobacter sp. Ni15; nickel resistance; plasmid pNi15; nrp gene Abstract: Abstract We isolated nickel-resistant bacterium from soil in order to identify a novel nickel resistance determinant. Using 16S rRNA gene sequencing, an isolate was identified as Enterobacter sp. Ni15. This species showed a medium-level (resistant to up to 10 mM) nickel resistance in nutrient-rich media. Enterobacter sp. Ni15 has a novel plasmid, pNi15, and an increased nickel resistance to Escherichia coli DH5[alpha]in trans. To isolate the nickel resistance gene from pNi15, the plasmid was digested with XbaI and its fragments were cloned into pBluescriptIISK(+). The clones were transferred into E. coli DH5[alpha]. The nickel resistance of the clones was then assayed. From these results, a pNi15100 isolate containing a 5328 bp XbaI fragment of pNi15 was identified and sequenced. The E. coli DH5[alpha] harboring the pNi15100 showed a resistance to up to 7 mM nickel. Using a subcloning analysis, we were able to identify the novel nickel resistance determinant: the nrp gene encoding the putative proteins NrpA and NrpB. Article History: Received 27 October 2005; revised 26 December 2005; accepted 27 December 2005. First published online 24 February 2006. Article note: Correspondence: Young-Keun Lee, Radiation Application Research Division, ARTI, Korea Atomic Energy Research Institute, Sinjeong-Dong 1266, Jeongeup, 580-185, Korea. Tel.: +82 63 570 3300; fax: +82 63 570 3309; e-mail: yklee@kaeri.re.kr