학술논문
Hsp70, in Combination with IL-15 and PD-1 Blocker, Interferes with The Induction of Cytotoxic NK Cells in Relapsed Acute Myeloid Leukemia Patients
Document Type
article
Author
Source
Cell Journal, Vol 25, Iss 2, Pp 92-101 (2023)
Subject
Language
English
ISSN
2228-5806
2228-5814
2228-5814
Abstract
Objective: Natural killer (NK) cells are critical immune cells for acute myeloid leukemia (AML) targeting. However,little is known about the relationship between using checkpoint inhibitors and heat shock protein 70 (Hsp70) as NK cellactivators to control AML. Therefore, the study aims to find the best formulation of Hsp70, human PD-1 (Programmedcell death protein 1) blocker, and interleukin 15 (IL-15) to activate NK cells against AML.Materials and Methods: In this experimental study, the NK cells were isolated from mononuclear cells (MNCs) byusing magnetic activation cell sorting (MACS) and were activated using the different combinations of Hsp70, PD-1blocker, and IL-15 and then followed by immunophenotyping, functional assays to estimate their killing potential, andevaluation of expression pattern of PRF1, PIK3CB, PD-1, AKT-1, FAS-L, TRAIL, and GER A and B.Results: The expression of PD-1 was significantly (P