학술논문
descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques
Document Type
article
Author
Kohei Otomo; Takaki Omura; Yuki Nozawa; Steven J. Edwards; Yukihiko Sato; Yuri Saito; Shigehiro Yagishita; Hitoshi Uchida; Yuki Watakabe; Kiyotada Naitou; Rin Yanai; Naruhiko Sahara; Satoshi Takagi; Ryohei Katayama; Yusuke Iwata; Toshiro Shiokawa; Yoku Hayakawa; Kensuke Otsuka; Haruko Watanabe-Takano; Yuka Haneda; Shigetomo Fukuhara; Miku Fujiwara; Takenobu Nii; Chikara Meno; Naoki Takeshita; Kenta Yashiro; Juan Marcelo Rosales Rocabado; Masaru Kaku; Tatsuya Yamada; Yumiko Oishi; Hiroyuki Koike; Yinglan Cheng; Keisuke Sekine; Jun-ichiro Koga; Kaori Sugiyama; Kenichi Kimura; Fuyuki Karube; Hyeree Kim; Ichiro Manabe; Tomomi Nemoto; Kazuki Tainaka; Akinobu Hamada; Hjalmar Brismar; Etsuo A. Susaki
Source
Nature Communications, Vol 15, Iss 1, Pp 1-19 (2024)
Subject
Language
English
ISSN
2041-1723
Abstract
Abstract Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM for cleared specimens), a low-cost ($20,000–50,000), low-expertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a three-dimensional manner. Academically open-sourced ( https://github.com/dbsb-juntendo/descSPIM ), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies.