학술논문
Successful weight regain attenuation by autologous fecal microbiota transplantation is associated with non-core gut microbiota changes during weight loss; randomized controlled trial
Document Type
article
Author
Omer Kamer; Ehud Rinott; Gal Tsaban; Alon Kaplan; Anat Yaskolka Meir; Hila Zelicha; Dan Knights; Kieran Tuohy; Francesca Fava; Matthias Uwe Scholz; Oren Ziv; Elad Rubin; Matthias Blüher; Michael Stumvoll; Uta Ceglarek; Karine Clément; Omry Koren; Frank B. Hu; Meir J. Stampfer; Dong D. Wang; Ilan Youngster; Iris Shai
Source
Gut Microbes, Vol 15, Iss 2 (2023)
Subject
Language
English
ISSN
19490976
1949-0984
1949-0976
1949-0984
1949-0976
Abstract
ABSTRACTWe previously reported that autologous-fecal-microbiota-transplantation (aFMT), following 6 m of lifestyle intervention, attenuated subsequent weight regain and insulin rebound for participants consuming a high-polyphenol green-Mediterranean diet. Here, we explored whether specific changes in the core (abundant) vs. non-core (low-abundance) gut microbiome taxa fractions during the weight-loss phase (0–6 m) were differentially associated with weight maintenance following aFMT. Eighty-two abdominally obese/dyslipidemic participants (age = 52 years; 6 m weightloss = −8.3 kg) who provided fecal samples (0 m, 6 m) were included. Frozen 6 m’s fecal samples were processed into 1 g, opaque and odorless aFMT capsules. Participants were randomly assigned to receive 100 capsules containing their own fecal microbiota or placebo over 8 m-14 m in ten administrations (adherence rate > 90%). Gut microbiome composition was evaluated using shotgun metagenomic sequencing. Non-core taxa were defined as ≤ 66% prevalence across participants. Overall, 450 species were analyzed. At baseline, 13.3% were classified as core, and Firmicutes presented the highest core proportion by phylum. During 6 m weight-loss phase, abundance of non-core species changed more than core species (P