학술논문
3D printed devices and infrastructure for liquid sample delivery at the European XFEL
Document Type
article
Author
Mohammad Vakili; Johan Bielecki; Juraj Knoška; Florian Otte; Huijong Han; Marco Kloos; Robin Schubert; Elisa Delmas; Grant Mills; Raphael de Wijn; Romain Letrun; Simon Dold; Richard Bean; Adam Round; Yoonhee Kim; Frederico A. Lima; Katerina Dörner; Joana Valerio; Michael Heymann; Adrian P. Mancuso; Joachim Schulz
Source
Journal of Synchrotron Radiation, Vol 29, Iss 2, Pp 331-346 (2022)
Subject
Language
English
ISSN
1600-5775
16005775
16005775
Abstract
The Sample Environment and Characterization (SEC) group of the European X-ray Free-Electron Laser (EuXFEL) develops sample delivery systems for the various scientific instruments, including systems for the injection of liquid samples that enable serial femtosecond X-ray crystallography (SFX) and single-particle imaging (SPI) experiments, among others. For rapid prototyping of various device types and materials, sub-micrometre precision 3D printers are used to address the specific experimental conditions of SFX and SPI by providing a large number of devices with reliable performance. This work presents the current pool of 3D printed liquid sample delivery devices, based on the two-photon polymerization (2PP) technique. These devices encompass gas dynamic virtual nozzles (GDVNs), mixing-GDVNs, high-viscosity extruders (HVEs) and electrospray conical capillary tips (CCTs) with highly reproducible geometric features that are suitable for time-resolved SFX and SPI experiments at XFEL facilities. Liquid sample injection setups and infrastructure on the Single Particles, Clusters, and Biomolecules and Serial Femtosecond Crystallography (SPB/SFX) instrument are described, this being the instrument which is designated for biological structure determination at the EuXFEL.