학술논문
High throughput single-cell detection of multiplex CRISPR-edited gene modifications
Document Type
article
Author
Source
Genome Biology, Vol 21, Iss 1, Pp 1-11 (2020)
Subject
Language
English
ISSN
1474-760X
Abstract
Abstract CRISPR-Cas9 gene editing has transformed our ability to rapidly interrogate the functional impact of somatic mutations in human cancers. Droplet-based technology enables the analysis of Cas9-introduced gene edits in thousands of single cells. Using this technology, we analyze Ba/F3 cells engineered to express single or multiplexed loss-of-function mutations recurrent in chronic lymphocytic leukemia. Our approach reliably quantifies mutational co-occurrences, zygosity status, and the occurrence of Cas9 edits at single-cell resolution.