학술논문
Salivary Microbial Dysbiosis Is Associated With Peri-Implantitis: A Case-Control Study in a Brazilian Population
Document Type
article
Author
Debora Pallos; Vanessa Sousa; Magda Feres; Belen Retamal-Valdes; Tsute Chen; Mike Curtis; Richardson Mondego Boaventura; Marcia Hiromi Tanaka; Gustavo Vargas da Silva Salomão; Louise Zanella; Tania Regina Tozetto-Mendoza; Gabriela Schwab; Lucas Augusto Moyses Franco; Ester Cerdeira Sabino; Paulo Henrique Braz-Silva; Jamil Awad Shibli
Source
Frontiers in Cellular and Infection Microbiology, Vol 11 (2022)
Subject
Language
English
ISSN
2235-2988
Abstract
Background and ObjectivesThe aim of this study was to examine the salivary microbiome in healthy peri-implant sites and those with peri-implantitis.MethodsSaliva samples were collected from 21 participants with healthy peri-implant sites and 21 participants with peri-implantitis. The V4 hypervariable region of the 16S rRNA gene was sequenced using the Ion Torrent PGM System (Ion 318™ Chip v2 400). The NGS analysis and composition of the salivary microbiome were determined by taxonomy assignment. Downstream bioinformatic analyses were performed in QIIME (v 1.9.1).ResultsClinical differences according to peri-implant condition status were found. Alpha diversity metrics revealed that the bacterial communities of participants with healthy peri-implant sites tended to have a richer microbial composition than individuals with peri-implantitis. In terms of beta diversity, bleeding on probing (BoP) may influence the microbial diversity. However, no clear partitioning was noted between the salivary microbiome of volunteers with healthy peri-implant sites or volunteers with peri-implantitis. The highest relative abundance of Stenotrophomonas, Enterococcus and Leuconostoc genus, and Faecalibacterium prausnitzii, Haemophilus parainfluenzae, Prevotella copri, Bacteroides vulgatus, and Bacteroides stercoris bacterial species was found in participants with peri-implantitis when compared with those with healthy peri-implant sites.ConclusionDifferences in salivary microbiome composition were observed between patients with healthy peri-implant sites and those with peri-implantitis. BoP could affect the diversity (beta diversity) of the salivary microbiome.