학술논문
T cell response to intact SARS-CoV-2 includes coronavirus cross-reactive and variant-specific components
Document Type
article
Author
Lichen Jing; Xia Wu; Maxwell P. Krist; Tien-Ying Hsiang; Victoria L. Campbell; Christopher L. McClurkan; Sydney M. Favors; Lawrence A. Hemingway; Charmie Godornes; Denise Q. Tong; Stacy Selke; Angela C. LeClair; Chu-Woo Pyo; Daniel E. Geraghty; Kerry J. Laing; Anna Wald; Michael Gale Jr.; David M. Koelle
Source
JCI Insight, Vol 7, Iss 6 (2022)
Subject
Language
English
ISSN
2379-3708
Abstract
SARS-CoV-2 provokes a robust T cell response. Peptide-based studies exclude antigen processing and presentation biology, which may influence T cell detection studies. To focus on responses to whole virus and complex antigens, we used intact SARS-CoV-2 and full-length proteins with DCs to activate CD8 and CD4 T cells from convalescent people. T cell receptor (TCR) sequencing showed partial repertoire preservation after expansion. Resultant CD8 T cells recognize SARS-CoV-2–infected respiratory tract cells, and CD4 T cells detect inactivated whole viral antigen. Specificity scans with proteome-covering protein/peptide arrays show that CD8 T cells are oligospecific per subject and that CD4 T cell breadth is higher. Some CD4 T cell lines enriched using SARS-CoV-2 cross-recognize whole seasonal coronavirus (sCoV) antigens, with protein, peptide, and HLA restriction validation. Conversely, recognition of some epitopes is eliminated for SARS-CoV-2 variants, including spike (S) epitopes in the Alpha, Beta, Gamma, and Delta variant lineages.