학술논문
UPLC-QE-MS结合UPLC表征绿萼梅不同发育时期的成分差异及抗氧化活性研究 / Compositional differences of Mume Flos at different developmental periods by UPLC-QE-MS and UPLC and their antioxidant activity
Document Type
Academic Journal
Author
Source
天然产物研究与开发 / Natural Product Research and Development. 35(11):1915-1928
Subject
Language
Chinese
ISSN
1001-6880
Abstract
梅花为蔷薇科植物梅Prunus mume(Sieb.)Sieb.et Zucc.的干燥花蕾,绿萼梅为临床入药的主要品种.该研究为明确不同发育时期绿萼梅的化学组成及差异,采用超高效液相色谱-四级杆静电场轨道阱质谱(UPLC-QE-MS)检测其化学组成,共检测得到328个化合物,包含67个黄酮、50个苯丙素、104个萜类、26个酚类、24个生物碱、11个有机酸及其衍生物、8个氨基酸衍生物及其他38个化合物.半定量结果显示绿萼梅中主要化学成分的含量随发育时期呈逐渐下降趋势.采用紫外-可见分光光度法对不同发育时期绿萼梅样品中总黄酮和总苯丙素含量进行测定,采用UPLC法测定不同发育时期8个指标性成分(新绿原酸、绿原酸、咖啡酸、芦丁、金丝桃苷、异槲皮苷、异绿原酸B、槲皮苷)含量,分析结果表明花蕾期与全开期之间成分含量差异显著,绿原酸可作为不同发育时期之间的差异质量标志物.DPPH自由基清除法检测不同发育时期绿萼梅的抗氧化活性,结果表明抗氧化能力与其成分含量密切相关,且随着花的发育逐渐减弱.该文结果可为梅花的化学成分动态变化规律和最佳采收期的确定提供分析方法和数据支撑.
Mume Flos is the dried flower bud of Prunus mume(Sieb.)Sieb.et Zucc.in the Rosaceae family,and the Prunus mume"Lve"is the main species used in clinical medicine.In this study,the chemical composition of Mume Flos was deter-mined by ultra-high performance liquid chromatography with quadrupole electrostatic field orbitrap mass spectrometry(UPLC-QE-MS)at different developmental periods.A total of 328 compounds were detected,including 67 flavonoids,50 phenylpro-panoids,104 terpenoids,26 phenols,24 alkaloids,11 organic acids and their derivatives,8 amino acid derivatives and 38 oth-ers.The semi-quantitative results showed a gradual decrease in the content of the main chemical constituents in Mume Flos with the developmental period.The content of the total flavonoid and phenylpropanoid was determined by UV-Vis spectropho-tometry in Mume Flos samples at different developmental periods.The content of eight indicator components(neochlorogenic acid,chlorogenic acid,caffeic acid,rutin,hyperoside,isoquercitrin,isochlorogenic acid B and quercitrin)at different develop-mental periods was determined by UPLC,the results of the analysis showed significant differences in the content of compo-nents between the bud period(HL)and the full-opening period(QK)and that chlorogenic acid could be used as a marker of differential quality between the different developmental periods.The results of the DPPH radical scavenging assay on the antioxidant activity of Mume Flos at different developmental periods showed that the antioxidant capacity was closely related to the content of its components and gradually decreased with the development of flowers.The results of this paper can provide analytical methods and data support for the dynamics of the chemical constituents and the determination of the optimum har-vesting period of Mume Flos.
Mume Flos is the dried flower bud of Prunus mume(Sieb.)Sieb.et Zucc.in the Rosaceae family,and the Prunus mume"Lve"is the main species used in clinical medicine.In this study,the chemical composition of Mume Flos was deter-mined by ultra-high performance liquid chromatography with quadrupole electrostatic field orbitrap mass spectrometry(UPLC-QE-MS)at different developmental periods.A total of 328 compounds were detected,including 67 flavonoids,50 phenylpro-panoids,104 terpenoids,26 phenols,24 alkaloids,11 organic acids and their derivatives,8 amino acid derivatives and 38 oth-ers.The semi-quantitative results showed a gradual decrease in the content of the main chemical constituents in Mume Flos with the developmental period.The content of the total flavonoid and phenylpropanoid was determined by UV-Vis spectropho-tometry in Mume Flos samples at different developmental periods.The content of eight indicator components(neochlorogenic acid,chlorogenic acid,caffeic acid,rutin,hyperoside,isoquercitrin,isochlorogenic acid B and quercitrin)at different develop-mental periods was determined by UPLC,the results of the analysis showed significant differences in the content of compo-nents between the bud period(HL)and the full-opening period(QK)and that chlorogenic acid could be used as a marker of differential quality between the different developmental periods.The results of the DPPH radical scavenging assay on the antioxidant activity of Mume Flos at different developmental periods showed that the antioxidant capacity was closely related to the content of its components and gradually decreased with the development of flowers.The results of this paper can provide analytical methods and data support for the dynamics of the chemical constituents and the determination of the optimum har-vesting period of Mume Flos.