학술논문
海洋生物 DNA 条形码研究现状与展望 / Current status and future prospect of DNA barcoding in marine biology
Document Type
Academic Journal
Author
林森杰; 王路; 郑连明; 董云伟; 柳淑芳; 丁少雄; 叶乃好; 曹文清; 庄志猛; Lin Senjie; Wang Lu; Zheng Lianming; Dong Yunwei; Liu Shufang; Ding Shaoxiong; Ye Naihao; Cao Wenqing; Zhuang Zhimeng
Source
海洋学报（中文版） / Acta Oceanologica Sinica. (12):1-17
Subject
Language
Chinese
ISSN
0253-4193
Abstract
海洋生物种类多样，分布广泛，具有复杂性、多样性和趋同性等特点，为了对物种进行更快速、准确地鉴定，急需在传统形态分类学基础上，建立并结合便捷准确的分子鉴定手段。DNA 条形码提供了可信息化的分类标准和有效的分类学手段，已成为近年来分类学与生物多样性研究中重要的技术依托。本文概述了 DNA 条形码当前的发展现状与趋势，并介绍了 DNA 条形码技术在主要海洋浮游植物（红藻、褐藻、绿藻、硅藻、甲藻）、无脊椎动物（海绵动物、刺胞动物、甲壳动物和软体动物等）和鱼类中的研究进展，以及不同条形码基因针对于不同生物类群的有效性和适用性，指出了目前条形码技术在各海洋类群中存在的主要问题，并对未来的相关工作做了展望，希望为今后我国的海洋生物DNA 条形码研究提供理论基础。
Marine organisms are highly diverse,widely distributed,with high complexity and homoplasy.To enable fast and accurate identification of species,it is imperative to establish molecular techniques,to complement the tradi-tional morphological metbodology.DNA barcoding provides digitalized criteria and effective means for species iden-tification,and is becoming an important technical tool in the research on taxonomy and biodiversity.In this review, we summarize the major recent progress and current trend in DNA barcoding,particularly as it applies to the fields of marine phytoplankton (Rhodophyte,Phaeophyta,Chlorophyta,Bacillariophyta and Dinophyta ),invertebrates (Spongia,Cnidaria,Custacea,Mollusca,etc.)and fish.We provide an overview of the deffectiveness and suitability of different barcoding markers in different groups of marine organisms.We also discuss current challenges and fu-ture prospects of marine DNA barcoding in hope to provide a framework for future marine DNA barcoding research in China.
Marine organisms are highly diverse,widely distributed,with high complexity and homoplasy.To enable fast and accurate identification of species,it is imperative to establish molecular techniques,to complement the tradi-tional morphological metbodology.DNA barcoding provides digitalized criteria and effective means for species iden-tification,and is becoming an important technical tool in the research on taxonomy and biodiversity.In this review, we summarize the major recent progress and current trend in DNA barcoding,particularly as it applies to the fields of marine phytoplankton (Rhodophyte,Phaeophyta,Chlorophyta,Bacillariophyta and Dinophyta ),invertebrates (Spongia,Cnidaria,Custacea,Mollusca,etc.)and fish.We provide an overview of the deffectiveness and suitability of different barcoding markers in different groups of marine organisms.We also discuss current challenges and fu-ture prospects of marine DNA barcoding in hope to provide a framework for future marine DNA barcoding research in China.