학술논문
不同贮藏条件对火麻种子生理生化及其活力的影响 / Effects of Different Storage Conditions on the Physiology and Biochemistry of Hemp Seeds and Their Viability
Document Type
Academic Journal
Author
罗欢; 郑其向; 杨永红; 许锦; 普瑞; 杨昆林; 杨昆彬; LUO Huan; ZHENG Qixiang; YANG Yonghong; XU Jin; PU Rui; YANG Kunlin; YANG Kunbin
Source
特产研究 / Special Wild Economic Animal and Plant Research. 46(1):31-36
Subject
Language
Chinese
ISSN
1001-4721
Abstract
本研究探究了不同含水量和贮藏温度处理下,火麻种子生理指标的变化情况,为火麻种质资源保存方面提供科学依据.以云南楚雄和黑龙江大庆的火麻种子为材料,分别设置6个不同含水量贮藏于7 ℃、-15 ℃、-30℃、-80℃和常温25 ℃左右条件下6个月,分析火麻种子过氧化氢酶(CAT)、过氧化物酶(POD)、脱氢酶(DHA)、丙二醛(MDA)、相对电导率、萌发率的变化情况.结果表明,不同含水量和不同贮藏温度对火麻种子的生理指标有影响,云南楚雄和黑龙江大庆的火麻种子均随种子含水量的增加其DHA活性呈先上升后降低趋势.大庆种子随种子含水量的增加其CAT活性呈上升趋势.楚雄种子和大庆种子MDA含量均随含水量增加有上升趋势.两地种子均随含水量的增加,种子的相对电导率增加.此外,与其他贮藏温度相比,楚雄种子在7 ℃下各含水量均维持较高萌发率.本研究结果表明,贮藏温度为7 ℃,楚雄种子含水量在5.13％～6.16％左右,大庆种子含水量在4.03％～5.26％左右时火麻种子活力较好,能提高种子品质,在火麻种子贮藏条件选择方面具有重要的参考价值.
To investigate the changes of physiological indexes of hemp seeds under different moisture content and storage temperature treat-ments,and to provide a scientific basis for the preservation of hemp germplasm resources.Hemp seeds from Chuxiong,Yunnan and Daqing,Heilongjiang were stored at 6 different moisture contents for 6 months at 7 ℃,-15 ℃,-30 ℃,-80 ℃ and about 25 ℃ at room temperature,and the changes of catalase(CAT),peroxidase(POD),dehydrogenase(DHA),malondialdehyde(MDA),relative conductivity and germination rate were analyzed.The results showed that the physiological indicators of hemp seeds were affected by different moisture contents and storage temperatures,and the DHA activity of both Chuxiong,Yunnan and Daqing,Heilongjiang seeds tended to increase and then decrease with in-creasing seed moisture content.The CAT activity of Daqing seeds tended to increase with the increase of seed moisture content.There was an increasing trend of MDA content with increasing moisture content in both varieties.The relative electrical conductivity of seeds increased with increasing moisture content in both varieties.In addition,compared with other storage temperatures,the seeds of Chuxiong maintained a higher germination rate at all moisture contents at 7 ℃.These results suggested that the storage temperature is 7 ℃,the moisture content of Chuxiong seeds is at about 5.13％-6.16％,the moisture content of Daqing seeds is at about 4.03％～5.26％of hemp seed viability is better,which can im-prove the quality of the seed,and has an important reference value in the selection of hemp seed storage conditions.
To investigate the changes of physiological indexes of hemp seeds under different moisture content and storage temperature treat-ments,and to provide a scientific basis for the preservation of hemp germplasm resources.Hemp seeds from Chuxiong,Yunnan and Daqing,Heilongjiang were stored at 6 different moisture contents for 6 months at 7 ℃,-15 ℃,-30 ℃,-80 ℃ and about 25 ℃ at room temperature,and the changes of catalase(CAT),peroxidase(POD),dehydrogenase(DHA),malondialdehyde(MDA),relative conductivity and germination rate were analyzed.The results showed that the physiological indicators of hemp seeds were affected by different moisture contents and storage temperatures,and the DHA activity of both Chuxiong,Yunnan and Daqing,Heilongjiang seeds tended to increase and then decrease with in-creasing seed moisture content.The CAT activity of Daqing seeds tended to increase with the increase of seed moisture content.There was an increasing trend of MDA content with increasing moisture content in both varieties.The relative electrical conductivity of seeds increased with increasing moisture content in both varieties.In addition,compared with other storage temperatures,the seeds of Chuxiong maintained a higher germination rate at all moisture contents at 7 ℃.These results suggested that the storage temperature is 7 ℃,the moisture content of Chuxiong seeds is at about 5.13％-6.16％,the moisture content of Daqing seeds is at about 4.03％～5.26％of hemp seed viability is better,which can im-prove the quality of the seed,and has an important reference value in the selection of hemp seed storage conditions.