학술논문
Molecular typing of Spanish species of Amanita by restriction analysis of the ITS region of the DNA
Document Type
Article
Source
Mycological Research; August 2002, Vol. 106 Issue: 8 p903-910, 8p
Subject
Language
ISSN
09537562
Abstract
The genetic relatedness among 29 collections belonging to 20 of the most common species of genus Amanita in the Iberian Peninsula have been studied by means of restriction analysis of the amplified ITS1-5.8S-ITS2 region of rDNA. Sixty restriction fragments were considered for the analysis. The main conclusion is that this technique could be useful to identify and characterize isolates from Amanita species. Although ARDRA did not show an appropriate level of discrimination to unambiguously infer phylogenetic relationships at or below the section level, some general trends could be outlined when the different haplotypes generated were compared by means of neighbour joining analysis. Thus, members from the same sections were frequently grouped together.