학술논문
Critical Role of a Sheath Phosphorylation Site On the Assembly and Function of an Atypical Type VI Secretion System*
Document Type
Article
Author
Source
Molecular and Cellular Proteomics (MCP Online); December 2019, Vol. 18 Issue: 12 p2418-2432, 15p
Subject
Language
ISSN
15359476; 15359484
Abstract
KCl stimulation triggers assembly of the Francisella T6SS in culture. Differential whole cell proteomics reveals that the amounts of the T6SS proteins remain unchanged upon KCl stimulation. A phosphoproteomic analysis identifies a phosphorylation on the T6SS sheath, at site Y139 of IglB. Our data demonstrate that site Y139 of IglB plays a critical role in T6SS biogenesis, providing novel mechanistic insight into the role of sheath phosphorylation in T6SS biogenesis.