학술논문
Label-Free Detection of Biomolecular Interactions Using BioLayer Interferometry for Kinetic Characterization
Document Type
Article
Author
Concepcion, Joy; Witte, Krista; Wartchow, Charles; Choo, Sae; Yao, Danfeng; Persson, Henrik; Wei, Jing; Li, Pu; Heidecker, Bettina; Ma, Weilei; Varma, Ram; Zhao, Lian-She; Perillat, Donald; Carricato, Greg; Recknor, Michael; Du, Kevin; Ho, Huddee; Ellis, Tim; Gamez, Juan; Howes, Michael; Phi-Wilson, Janette; Lockard, Scott; Zuk, Robert; Tan, Hong
Source
Combinatorial Chemistry & High Throughput Screening; September 2009, Vol. 12 Issue: 8 p791-800, 10p
Subject
Language
ISSN
13862073
Abstract
The analysis of biomolecular interactions is key in the drug development process. Label-free biosensor methods provide information on binding, kinetics, concentration, and the affinity of an interaction. These techniques provide real-time monitoring of interactions between an immobilized ligand (such as a receptor) to an analyte in solution without the use of labels. Advances in biosensor design and detection using BioLayer Interferometry (BLI) provide a simple platform that enables label-free monitoring of biomolecular interactions without the use of flow cells. We review the applications of BLI in a wide variety of research and development environments for quantifying antibodies and proteins and measuring kinetics parameters.