부산대학교 도서관

To obtain guidelines for the best practice characterization of protein complexes using co-fractionation mass spectrometry (CF-MS), novel and published Saccharomyces cerevisiaeCF-MS data sets are thoroughly evaluated using high proteome coverage libraries of yeast gold standard complexes. It is found that near-maximum recall of complexes can be achieved with fewer samples than published studies, several similarity scoring metrics – such as Spearman and Kendall correlations – are underutilized, and genomic data integration is generally unsuitable for predicting novel complexes in model organisms.