부산대학교 도서관

During routine histological examination of tissues from mortality events of anadromous Brook Trout Salvelinus fontinalisfrom Prince Edward Island (PEI), Canada, myxospores consistent with Myxoboluswere observed infecting the central nervous system. The objective of this study was to identify the species of Myxobolusinfecting the nervous system of anadromous Brook Trout from PEI, Canada. Myxospore morphology, small subunit (SSU) ribosomal DNA (rDNA) sequence data, and histology were used to identify myxospores isolated from infected Brook Trout. Myxospore measurements from the PEI samples matched those reported in the description of Myxobolus neurofontinalisfrom North Carolina. A 1057‐bp fragment of the SSU rDNA from myxospores collected from Brook Trout in PEI was identical to an isolate of M. neurofontinalis(MN191598) collected previously from the type locality, New River basin, North Carolina. Histological sections confirmed infections were intercellular in the central nervous system. Minimal host response was observed, with only sparse mononuclear inflammatory infiltrates present at the periphery of and within dispersed myxospores, suggesting that infections are not pathogenic to Brook Trout. Myxospores were identified as M. neurofontinalis, which was previously described from the central nervous system of Brook Trout from the New River basin, North Carolina, USA. This constitutes the first time M. neurofontinalishas been documented outside of the New River basin in North Carolina. Impact statementThis study documents Myxobolus neurofontinalisoutside of North Carolina for the first time and, given the morphological similarities to Myxobolus cerebralis, the causative agent of salmonid whirling disease, highlights the importance of detailed morphological diagnosis coupled with nucleotide sequence data to myxozoan disease diagnostics. This study documents Myxobolus neurofontinalisoutside of North Carolina for the first time and, given the morphological similarities to Myxobolus cerebralis, the causative agent of salmonid whirling disease, highlights the importance of detailed morphological diagnosis coupled with nucleotide sequence data to myxozoan disease diagnostics.