학술논문
Visualization of dendritic cells' responses in atopic dermatitis: Preventing effect of emollient.
Document Type
Editorial & Opinion
Author
Mias C; Skin Research Center, Pierre Fabre Dermo-Cosmétique, Toulouse, France.; Le Digabel J; Skin Research Center, Pierre Fabre Dermo-Cosmétique, Toulouse, France.; Filiol J; Skin Research Center, Pierre Fabre Dermo-Cosmétique, Toulouse, France.; Gontier E; Bordeaux Imaging Center-UMS 3420 CNRS/Université de Bordeaux-US4 INSERM, Bordeaux, France.; Gravier E; Skin Research Center, Pierre Fabre Dermo-Cosmétique, Toulouse, France.; Villaret A; Skin Research Center, Pierre Fabre Dermo-Cosmétique, Toulouse, France.; Nocera T; Skin Research Center, Pierre Fabre Dermo-Cosmétique, Toulouse, France.; Questel E; Skin Research Center, Pierre Fabre Dermo-Cosmétique, Toulouse, France.; Rossi AB; Skin Research Center, Pierre Fabre Dermo-Cosmétique, Toulouse, France.; Redoulès D; Skin Research Center, Pierre Fabre Dermo-Cosmétique, Toulouse, France.; Josse G; Skin Research Center, Pierre Fabre Dermo-Cosmétique, Toulouse, France.
Source
Publisher: Munksgaard Country of Publication: Denmark NLM ID: 9301549 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-0625 (Electronic) Linking ISSN: 09066705 NLM ISO Abbreviation: Exp Dermatol Subsets: MEDLINE
Subject
Language
English
Abstract
Atopic dermatitis (AD) is a chronic and multifactorial inflammatory skin disease involving various dendritic cells such as epidermal Langerhans cells (LC) and inflammatory dendritic epidermal cells (IDECs). Most of the clinical studies was performed on isolated cells, and thus, it would be useful to characterize directly on the human epidermal tissue the first cellular events occurred during the AD. The suction blister method was used to obtain whole epidermis samples and interstitial cutaneous fluids. Employing multiphoton microscopy, we analyzed the early dynamic behavior of inflammatory cells using Dermatophagoides pteronyssinus atopy patch test (Derp-APT) and evaluated the effects of emollient pre-application. Derp-APT application provoked rapid and strong infiltration of IDECs, and proliferation and activation of LC in the AD subjects' epidermis. Moreover, emollient pre-application strengthened the defective skin barrier and had positive effects on inflammatory cells' behavior, characterized by the complete inhibition of IDEC influx and the presence of immature LC.
(© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
(© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)