학술논문
Cytokine Release Patterns in Mixed Lymphocyte Culture (MLC) of T-Cells with Dendritic Cells (DC) Generated from AML Blasts Contribute to Predict anti-Leukaemic T-Cell Reactions and Patients' Response to Immunotherapy.
Document Type
Academic Journal
Author
Fischbacher D; a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.; Merle M; a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.; Liepert A; a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.; Grabrucker C; a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.; Kroell T; a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.; Kremser A; a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.; Dreyßig J; a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.; Freudenreich M; a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.; Schuster F; b Department for pediatric Haematology and Oncology , University Hospital of Düsseldorf , Düsseldorf , Germany.; Borkhardt A; b Department for pediatric Haematology and Oncology , University Hospital of Düsseldorf , Düsseldorf , Germany.; Kraemer D; c Municipal Hospital Oldenburg , Oldenburg , Germany.; Koehne CH; c Municipal Hospital Oldenburg , Oldenburg , Germany.; Kolb HJ; a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.; d Helmholtz Center, Munich, Clinical cooperative group Human Cell Transplantation (CCG-HCT) , Munich , Germany.; Schmid C; e Municipal Hospital Augsburg , Augsburg , Germany.; Schmetzer HM; a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.; d Helmholtz Center, Munich, Clinical cooperative group Human Cell Transplantation (CCG-HCT) , Munich , Germany.
Source
Publisher: Taylor & Francis Country of Publication: England NLM ID: 101096596 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1543-5180 (Electronic) Linking ISSN: 15435180 NLM ISO Abbreviation: Cell Commun Adhes Subsets: MEDLINE
Subject
Language
English
Abstract
To enlighten interactions between autologous, allogeneic or T-cells from patients after stem cell transplantation with leukaemia-derived-dendritic-cells containing dendritic cells or blast containing mononuclear cells (n = 21, respectively), we determined cytokine-concentrations (interleukin 2, 4, 6, 10, tumor-necrosis-factor-α, interferon-γ) in supernatants of mixed-lymphocyte-culture and in serum (n = 16) of 20 patients with acute myeloid leukaemia and three patients with myelodysplastic syndromes by cytometric-bead-assay. We correlated our data with lytic capabilities of stimulated T-cells in a fluorolysis-assay and clinical data: Dendritic-cell-/mononuclear-cell-stimulation of T-cells resulted in increased cytokine-levels in culture-medium compared to serum. There were no significant differences between cytokine-patterns of cases with/without lytic T-cell-activity, response to immunotherapy (stem cell transplantation/donor-lymphocyte-infusion) or graft-versus-host-disease. However, some predictive cytokine-cut-off-values for antileukaemic T-cell-activity, patients' response to immunotherapy and graft-versus-host-disease could be defined. Cytokine-profiles alone, without functional assays, are no useful tool to predict antileukaemic T-cell-function, although they can indicate lytic T-cell-activity, patients' response to immunotherapy and graft-versus-host-disease.