학술논문
Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection.
Document Type
Academic Journal
Author
Udukala DN; Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA.; Wang H; Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA.; Wendel SO; Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA.; Malalasekera AP; Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA.; Samarakoon TN; Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA.; Yapa AS; Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA.; Abayaweera G; Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA.; Basel MT; Kansas State University, Department of Anatomy & Physiology, 228 Coles Hall, Manhattan, KS, USA.; Maynez P; Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA.; Ortega R; Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA.; Toledo Y; Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA.; Bossmann L; Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA.; Robinson C; Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA.; Janik KE; Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA.; Koper OB; Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA.; Li P; Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA.; Motamedi M; The University of Texas Medical Branch, 301 University Boulevard, Galveston, TX, USA.; Higgins DA; Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA.; Gadbury G; Kansas State University, Department of Statistics, 101 Dickens Hall, Manhattan, KS, USA.; Zhu G; The First Affiliated Hospital of Kunming Medical University, Department of Nuclear Medicine, 295 Xichang Road, Kunming, Yunnan, PR China.; Troyer DL; Kansas State University, Department of Anatomy & Physiology, 228 Coles Hall, Manhattan, KS, USA.; Bossmann SH; Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA.
Source
Publisher: Beilstein-Institut zur Förderung der Chemischen Wissenschaften Country of Publication: Germany NLM ID: 101551563 Publication Model: eCollection Cited Medium: Print ISSN: 2190-4286 (Print) Linking ISSN: 21904286 NLM ISO Abbreviation: Beilstein J Nanotechnol Subsets: PubMed not MEDLINE
Subject
Language
English
ISSN
2190-4286
Abstract
Proteases, including matrix metalloproteinases (MMPs), tissue serine proteases, and cathepsins (CTS) exhibit numerous functions in tumor biology. Solid tumors are characterized by changes in protease expression levels by tumor and surrounding tissue. Therefore, monitoring protease levels in tissue samples and liquid biopsies is a vital strategy for early cancer detection. Water-dispersable Fe/Fe3O4-core/shell based nanoplatforms for protease detection are capable of detecting protease activity down to sub-femtomolar limits of detection. They feature one dye (tetrakis(carboxyphenyl)porphyrin (TCPP)) that is tethered to the central nanoparticle by means of a protease-cleavable consensus sequence and a second dye (Cy 5.5) that is directly linked. Based on the protease activities of urokinase plasminogen activator (uPA), MMPs 1, 2, 3, 7, 9, and 13, as well as CTS B and L, human breast cancer can be detected at stage I by means of a simple serum test. By monitoring CTS B and L stage 0 detection may be achieved. This initial study, comprised of 46 breast cancer patients and 20 apparently healthy human subjects, demonstrates the feasibility of protease-activity-based liquid biopsies for early cancer diagnosis.