학술논문
2DE maps in the discovery of human autoimmune kidney diseases: the case of membranous glomerulonephritis.
Document Type
Academic Journal
Author
Bruschi M; Laboratory on Pathophysiology of Uremia, Istituto Giannina Gaslini, Largo G. Gaslini 5, Genoa, Italy.; Santucci L; Ghiggeri GM; Candiano G
Source
Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
Subject
Language
English
Abstract
The identification of antigens in the autoimmune diseases is a primary point to elucidate the pathogenesis of disease. Here, we propose an "in vivo" proteomics approach to identify the antigens of auto-antibodies in membranous glomerulonephritis. In this approach, podocyte proteins resolved by two-dimensional electrophoresis were semidry blotted to nitrocellulose membrane. Then the antibodies eluted from microdissected glomeruli and serum samples were used as a probe for the detection of podocyte antigens and characterized by means of mass spectrometry. These combined methods allowed us to identify six new antigens in membranous glomerulonephritis.