학술논문
Depth-Specific Hypoxic Responses to Spreading Depolarizations in Gyrencephalic Swine Cortex Unveiled by Photoacoustic Imaging.
Document Type
Academic Journal
Author
Santos E; Department of Neurosurgery, Evangelisches Krankenhaus Oldenburg, Carl Von Ossietzky University of Oldenburg, Marienstraße 11, 26121, Oldenburg, Germany.; Department of Neurosurgery, Heidelberg University Hospital, Ruprecht-Karls-University Heidelberg, Heidelberg, Germany.; Spine Center Stuttgart, Paulinenhilfe, Diakonie-Klinikum Stuttgart, Stuttgart, Germany.; Lopez-Navarro JM; Department of Neurosurgery, Evangelisches Krankenhaus Oldenburg, Carl Von Ossietzky University of Oldenburg, Marienstraße 11, 26121, Oldenburg, Germany.; Suarez-Gutierrez MA; Department of Neurosurgery, Evangelisches Krankenhaus Oldenburg, Carl Von Ossietzky University of Oldenburg, Marienstraße 11, 26121, Oldenburg, Germany.; Holzwarth N; Division of Intelligent Medical Systems, German Cancer Research Center (DKFZ), Heidelberg, Germany.; Albiña-Palmarola P; Neuroradiologische Klinik, Klinikum Stuttgart, Stuttgart, Germany.; Medical Faculty, University Duisburg-Essen, Essen, Germany.; Department of Anatomy, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile.; Kirchner T; Institut Für Physik, Martin-Luther-Universität Halle-Wittenberg, Halle (Saale), Germany.; Hernandez-Aguilera A; Department of Neurosurgery, Heidelberg University Hospital, Ruprecht-Karls-University Heidelberg, Heidelberg, Germany.; Fernandez-Amador JA; Department of Pathology, Nostra Senyora de Meritxell, Escaldes Engordany, Les Escaldes, Andorra.; Vazifehdan F; Spine Center Stuttgart, Paulinenhilfe, Diakonie-Klinikum Stuttgart, Stuttgart, Germany.; Woitzik J; Department of Neurosurgery, Evangelisches Krankenhaus Oldenburg, Carl Von Ossietzky University of Oldenburg, Marienstraße 11, 26121, Oldenburg, Germany.; Maier-Hein L; Division of Intelligent Medical Systems, German Cancer Research Center (DKFZ), Heidelberg, Germany.; Sanchez-Porras R; Department of Neurosurgery, Evangelisches Krankenhaus Oldenburg, Carl Von Ossietzky University of Oldenburg, Marienstraße 11, 26121, Oldenburg, Germany. renan_md@hotmail.com.
Source
Publisher: Springer Country of Publication: United States NLM ID: 101517297 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1868-601X (Electronic) Linking ISSN: 18684483 NLM ISO Abbreviation: Transl Stroke Res Subsets: MEDLINE
Subject
Language
English
Abstract
Spreading depolarizations (SDs) are a marker of brain injury and have a causative effect on ischemic lesion progression. The hemodynamic responses elicited by SDs are contingent upon the metabolic integrity of the affected tissue, with vasoconstrictive reactions leading to pronounced hypoxia often indicating poor outcomes. The stratification of hemodynamic responses within different cortical layers remains poorly characterized. This pilot study sought to elucidate the depth-specific hemodynamic changes in response to SDs within the gray matter of the gyrencephalic swine brain. Employing a potassium chloride-induced SD model, we utilized multispectral photoacoustic imaging (PAI) to estimate regional cerebral oxygen saturation (rcSO2%) changes consequent to potassium chloride-induced SDs. Regions of interest were demarcated at three cortical depths covering up to 4 mm. Electrocorticography (ECoG) strips were placed to validate the presence of SDs. Through PAI, we detected 12 distinct rcSO2% responses, which corresponded with SDs detected in ECoG. Notably, a higher frequency of hypoxic responses was observed in the deeper cortical layers compared to superficial layers, where hyperoxic and mixed responses predominated (p < 0.001). This data provides novel insights into the differential oxygenation patterns across cortical layers in response to SDs, underlining the complexity of cerebral hemodynamics post-injury.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)