학술논문
Redefining histological cell counts using a standardized method: The Leuven Intestinal Counting Protocol.
Document Type
Academic Journal
Author
Ceulemans M; Translational Research Center for Gastrointestinal Disorders (TARGID), Department of Chronic Diseases and Metabolism (ChroMeta), Katholieke Universiteit Leuven, Leuven, Belgium.; Huyghe P; Translational Research Center for Gastrointestinal Disorders (TARGID), Department of Chronic Diseases and Metabolism (ChroMeta), Katholieke Universiteit Leuven, Leuven, Belgium.; De Hertogh G; Laboratory of Translational Cell & Tissue Research, Department of Imaging & Pathology, Katholieke Universiteit Leuven, Leuven, Belgium.; Cameron R; College of Health, Medicine and Wellbeing, University of Newcastle, Newcastle, Australia.; National Health and Medical Research Council Centre for Research Excellence in Digestive Health, Newcastle, Australia.; Immune Health Research Program, Hunter Medical Research Institute, Newcastle, Australia.; Schol J; Translational Research Center for Gastrointestinal Disorders (TARGID), Department of Chronic Diseases and Metabolism (ChroMeta), Katholieke Universiteit Leuven, Leuven, Belgium.; Department of Gastroenterology and Hepatology, University Hospitals Leuven, Leuven, Belgium.; Burns GL; College of Health, Medicine and Wellbeing, University of Newcastle, Newcastle, Australia.; National Health and Medical Research Council Centre for Research Excellence in Digestive Health, Newcastle, Australia.; Immune Health Research Program, Hunter Medical Research Institute, Newcastle, Australia.; Keely S; College of Health, Medicine and Wellbeing, University of Newcastle, Newcastle, Australia.; National Health and Medical Research Council Centre for Research Excellence in Digestive Health, Newcastle, Australia.; Immune Health Research Program, Hunter Medical Research Institute, Newcastle, Australia.; Wauters L; Translational Research Center for Gastrointestinal Disorders (TARGID), Department of Chronic Diseases and Metabolism (ChroMeta), Katholieke Universiteit Leuven, Leuven, Belgium.; Department of Gastroenterology and Hepatology, University Hospitals Leuven, Leuven, Belgium.; Tack J; Translational Research Center for Gastrointestinal Disorders (TARGID), Department of Chronic Diseases and Metabolism (ChroMeta), Katholieke Universiteit Leuven, Leuven, Belgium.; Department of Gastroenterology and Hepatology, University Hospitals Leuven, Leuven, Belgium.; Talley NJ; College of Health, Medicine and Wellbeing, University of Newcastle, Newcastle, Australia.; National Health and Medical Research Council Centre for Research Excellence in Digestive Health, Newcastle, Australia.; Immune Health Research Program, Hunter Medical Research Institute, Newcastle, Australia.; Vanuytsel T; Translational Research Center for Gastrointestinal Disorders (TARGID), Department of Chronic Diseases and Metabolism (ChroMeta), Katholieke Universiteit Leuven, Leuven, Belgium.; Department of Gastroenterology and Hepatology, University Hospitals Leuven, Leuven, Belgium.
Source
Publisher: Wolters Kluwer Health Country of Publication: United States NLM ID: 101532142 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2155-384X (Electronic) Linking ISSN: 2155384X NLM ISO Abbreviation: Clin Transl Gastroenterol Subsets: MEDLINE
Subject
Language
English
Abstract
Objectives: The diagnosis of eosinophilic gastrointestinal diseases is largely based on mucosal eosinophil counts, but thresholds and normal ranges beyond the esophagus are debated, calling for much-needed methodological standardization. We aimed to develop a standardized workflow for duodenal cell quantification and estimate duodenal eosinophil and mast cell numbers in healthy controls.
Methods: Software-based histological cell quantification using free-sized or fixed-sized regions was developed and applied to digitized hematoxylin and eosin (H&E) stained slides from 58 individuals (healthy controls (HC) and functional dyspepsia (FD) patients). Intraclass correlation coefficients (ICC) compared interrater reliability between software-based and microscopic quantification. Reproducibility of the software-based method was validated in an independent cohort of 37 control and FD subjects. Eosinophil identification on H&E staining was compared to immunohistochemistry (IHC). Normal eosinophil (H&E) and mast cell (cKit) ranges were determined in 70 adult HC.
Results: Eosinophil quantification on digitized slides demonstrated excellent (ICC: 0.909) and significantly improved reproducibility over microscopic evaluation (ICC: 0.796, P = .0014), validated in an independent cohort (ICC: 0.910). Duodenal eosinophils were more abundant around crypts than in villi (P < .0001), while counts were similar on matched H&E and IHC stained slides (P = .55). Mean ± standard deviation (95th percentile) duodenal eosinophils and mast cells in HC were 228.8/mm2 ± 94.7 (402.8/mm2) and 419.5/mm2 ± 132.2 (707.6/mm2), respectively.
Conclusion: We developed and validated a standardized approach to duodenal histological cell quantification, generalizable to various mucosal cell types. Implementation of software-based quantification identified 400 eosinophils/mm2 and 700 mast cells/mm2 as thresholds for abnormal duodenal infiltration.
(Copyright © 2024 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of The American College of Gastroenterology.)
Methods: Software-based histological cell quantification using free-sized or fixed-sized regions was developed and applied to digitized hematoxylin and eosin (H&E) stained slides from 58 individuals (healthy controls (HC) and functional dyspepsia (FD) patients). Intraclass correlation coefficients (ICC) compared interrater reliability between software-based and microscopic quantification. Reproducibility of the software-based method was validated in an independent cohort of 37 control and FD subjects. Eosinophil identification on H&E staining was compared to immunohistochemistry (IHC). Normal eosinophil (H&E) and mast cell (cKit) ranges were determined in 70 adult HC.
Results: Eosinophil quantification on digitized slides demonstrated excellent (ICC: 0.909) and significantly improved reproducibility over microscopic evaluation (ICC: 0.796, P = .0014), validated in an independent cohort (ICC: 0.910). Duodenal eosinophils were more abundant around crypts than in villi (P < .0001), while counts were similar on matched H&E and IHC stained slides (P = .55). Mean ± standard deviation (95th percentile) duodenal eosinophils and mast cells in HC were 228.8/mm2 ± 94.7 (402.8/mm2) and 419.5/mm2 ± 132.2 (707.6/mm2), respectively.
Conclusion: We developed and validated a standardized approach to duodenal histological cell quantification, generalizable to various mucosal cell types. Implementation of software-based quantification identified 400 eosinophils/mm2 and 700 mast cells/mm2 as thresholds for abnormal duodenal infiltration.
(Copyright © 2024 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of The American College of Gastroenterology.)