학술논문
Electrical signature of heterogeneous human mesenchymal stem cells.
Document Type
Academic Journal
Author
Tsai T; Department of Chemical and Biomolecular Engineering, University of California Irvine, Irvine, California, USA.; Sue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, California, USA.; Vyas PD; Department of Chemical and Biomolecular Engineering, University of California Irvine, Irvine, California, USA.; Crowell LL; Department of Chemical and Biomolecular Engineering, University of California Irvine, Irvine, California, USA.; Sue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, California, USA.; Tran M; Department of Chemical and Biomolecular Engineering, University of California Irvine, Irvine, California, USA.; Sue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, California, USA.; Ward DW; Department of Chemical and Biomolecular Engineering, University of California Irvine, Irvine, California, USA.; Qin Y; Department of Chemical and Biomolecular Engineering, University of California Irvine, Irvine, California, USA.; Castro A; Department of Chemical and Biomolecular Engineering, University of California Irvine, Irvine, California, USA.; Adams TNG; Department of Chemical and Biomolecular Engineering, University of California Irvine, Irvine, California, USA.; Sue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, California, USA.; Department of Biomedical Engineering, University of California Irvine, Irvine, California, USA.
Source
Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 8204476 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-2683 (Electronic) Linking ISSN: 01730835 NLM ISO Abbreviation: Electrophoresis Subsets: MEDLINE
Subject
Language
English
Abstract
Human mesenchymal stem cells (hMSCs) have gained traction in transplantation therapy due to their immunomodulatory, paracrine, immune-evasive, and multipotent differentiation potential. The inherent heterogeneity of hMSCs poses a challenge for therapeutic treatments and necessitates the identification of robust biomarkers to ensure reproducibility in both in vivo and in vitro experiments. In this study, we utilized dielectrophoresis (DEP), a label-free electrokinetic phenomenon, to investigate the heterogeneity of hMSCs derived from bone marrow (BM) and adipose tissue (AD). The electrical properties of BM-hMSCs were compared to homogeneous mouse fibroblasts (NIH-3T3), human fibroblasts (WS1), and human embryonic kidney cells (HEK-293). The DEP profile of BM-hMSCs differed most from HEK-293 cells. We compared the DEP profiles of BM-hMSCs and AD-hMSCs and found that they have similar membrane capacitances, differing cytoplasm conductivity, and transient slopes. Inducing both populations to differentiate into adipocyte and osteoblast cells revealed that they behave differently in response to differentiation-inducing cytokines. Histology and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analyses of the differentiation-related genes revealed differences in heterogeneity between BM-hMSCs and AD-hMSCs. The differentiation profiles correlate well with the DEP profiles developed and indicate differences in the heterogeneity of BM-hMSCs and AD-hMSCs. Our results demonstrate that using DEP, membrane capacitance, cytoplasm conductivity, and transient slope can uniquely characterize the inherent heterogeneity of hMSCs to guide robust and reproducible stem cell transplantation therapies.
(© 2024 The Authors. Electrophoresis published by Wiley‐VCH GmbH.)
(© 2024 The Authors. Electrophoresis published by Wiley‐VCH GmbH.)