학술논문
Metatranscriptomics sheds light on the links between the functional traits of fungal guilds and ecological processes in forest soil ecosystems.
Document Type
Academic Journal
Author
Auer L; Université de Lorraine, INRAE, UMR Interactions Arbres-Microorganismes, Nancy, F-54000, France.; Buée M; Université de Lorraine, INRAE, UMR Interactions Arbres-Microorganismes, Nancy, F-54000, France.; Fauchery L; Université de Lorraine, INRAE, UMR Interactions Arbres-Microorganismes, Nancy, F-54000, France.; Lombard V; Architecture et Fonction des Macromolécules Biologiques, CNRS and Aix-Marseille Université, Marseille, 13288, France.; INRAE, USC1408 Architecture et Fonction des Macromolécules Biologiques, Marseille, 13009, France.; Barry KW; US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Clum A; US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Copeland A; US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Daum C; US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Foster B; US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; LaButti K; US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Singan V; US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Yoshinaga Y; US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Martineau C; Laurentian Forestry Centre, Natural Resources Canada, Canadian Forest Service, Quebec, G1V4C7, QC, Canada.; Alfaro M; Institute for Multidisciplinary Research in Applied Biology (IMAB), Public University of Navarra (UPNA), Pamplona, 31006, Spain.; Castillo FJ; Institute for Multidisciplinary Research in Applied Biology (IMAB), Public University of Navarra (UPNA), Pamplona, 31006, Spain.; Imbert JB; Institute for Multidisciplinary Research in Applied Biology (IMAB), Public University of Navarra (UPNA), Pamplona, 31006, Spain.; Ramírez L; Institute for Multidisciplinary Research in Applied Biology (IMAB), Public University of Navarra (UPNA), Pamplona, 31006, Spain.; Castanera R; Institute for Multidisciplinary Research in Applied Biology (IMAB), Public University of Navarra (UPNA), Pamplona, 31006, Spain.; Pisabarro AG; Institute for Multidisciplinary Research in Applied Biology (IMAB), Public University of Navarra (UPNA), Pamplona, 31006, Spain.; Finlay R; Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, Uppsala, 75007, Sweden.; Lindahl B; Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, Uppsala, 75007, Sweden.; Olson A; Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, Uppsala, 75007, Sweden.; Séguin A; Laurentian Forestry Centre, Natural Resources Canada, Canadian Forest Service, Quebec, G1V4C7, QC, Canada.; Kohler A; Université de Lorraine, INRAE, UMR Interactions Arbres-Microorganismes, Nancy, F-54000, France.; Henrissat B; DTU Bioengineering, Denmarks Tekniske Universitet, Copenhagen, 2800, Denmark.; Department of Biological Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.; Grigoriev IV; US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA, 94720, USA.; Martin FM; Université de Lorraine, INRAE, UMR Interactions Arbres-Microorganismes, Nancy, F-54000, France.
Source
Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE
Subject
Language
English
Abstract
Soil fungi belonging to different functional guilds, such as saprotrophs, pathogens, and mycorrhizal symbionts, play key roles in forest ecosystems. To date, no study has compared the actual gene expression of these guilds in different forest soils. We used metatranscriptomics to study the competition for organic resources by these fungal groups in boreal, temperate, and Mediterranean forest soils. Using a dedicated mRNA annotation pipeline combined with the JGI MycoCosm database, we compared the transcripts of these three fungal guilds, targeting enzymes involved in C- and N mobilization from plant and microbial cell walls. Genes encoding enzymes involved in the degradation of plant cell walls were expressed at a higher level in saprotrophic fungi than in ectomycorrhizal and pathogenic fungi. However, ectomycorrhizal and saprotrophic fungi showed similarly high expression levels of genes encoding enzymes involved in fungal cell wall degradation. Transcripts for N-related transporters were more highly expressed in ectomycorrhizal fungi than in other groups. We showed that ectomycorrhizal and saprotrophic fungi compete for N in soil organic matter, suggesting that their interactions could decelerate C cycling. Metatranscriptomics provides a unique tool to test controversial ecological hypotheses and to better understand the underlying ecological processes involved in soil functioning and carbon stabilization.
(© 2023 His Majesty the King in Right of Canada and The Authors. New Phytologist © 2023 New Phytologist Foundation Reproduced with the permission of the Minister of Natural Resources Canada. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.)
(© 2023 His Majesty the King in Right of Canada and The Authors. New Phytologist © 2023 New Phytologist Foundation Reproduced with the permission of the Minister of Natural Resources Canada. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.)